PCR effects of melting temperature adjustment of individual primers in degenerate primer pools
نویسندگان
چکیده
منابع مشابه
Selecting Degenerate Multiplex PCR Primers
Single Nucleotide Polymorphism (SNP) Genotyping is an important molecular genetics technique in the early stages of producing results that will be useful in the medical field. One of the proposed methods for performing SNP Genotyping requires amplifying regions of DNA surrounding a large number of SNP loci. In order to automate a portion of this method and make the use of SNP Genotyping more wi...
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UNLABELLED Designed degenerate primers unlike conventional primers are superior in matching and amplification of large number of genes, from related gene families. DPPrimer tool was designed to predict primers for PCR amplification of homologous gene from related or diverse plant species. The key features of this tool include platform independence and user friendliness in primer design. Embedde...
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متن کاملComputer program for calculating the melting temperature of degenerate oligonucleotides used in PCR or hybridization.
Degenerate primers or probes have been widely used in molecular cloning, but the calculation of their melting temperatures could not simply be done using thermodynamic parameters because of degeneracy and the lack of a computer program. We present here a simple computer program named dPrimer for the calculation of melting temperature of degenerate oligonucleotides based on the nearest-neighbor ...
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ژورنال
عنوان ژورنال: PeerJ
سال: 2019
ISSN: 2167-8359
DOI: 10.7717/peerj.6570